Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LOX1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LOX1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LOX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LOX1 can be calculated.
Background: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a 270-amino acid protein that in humans is encoded by the OLR1 gene, which spans approximately 15 kb and contains 6 exons. LOX1 is expressed in vascular-rich organs but not in lymphocytes, and it belongs to the C-type lectin superfamily. This protein binds, internalizes and degrades oxidized low-density lipoprotein. It may be involved in the regulation of Fas-induced apoptosis, and it may also play a role as a scavenger receptor. Mutations of the OLR1 gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease